ICSI is often used for couples with sperm problems. Sometimes it is also used for older couples or for those with failed IVF attempts. In males, where there is no sperms in ejaculate, the sperms retrieved surgically (PESA/ TESA/ MICROTESE) can be used for ICSI. In ICSI, a single sperm is injected into a mature egg. Then the embryo is transferred to the uterus.